Ukraine parliament names speaker Turchynov interim president
Parliament also voted to seize Mr Yanukovych's luxury estate near Kiev, which protesters entered on Saturday.
The whereabouts of Mr Yanukovych, who described parliament's decision to vote him out as a coup, remain unclear.
Thousands of protesters remain in Independence Square where the atmosphere is described as calm.
Late on Saturday, former Prime Minister Yulia Tymoshenko, freed from detention in the eastern city of Kharkiv after a vote in parliament, urged opposition supporters in Independence Square to continue protesting.
Her release was one of the conditions of the EU-Ukraine trade pact that President Yanukovych rejected last year - triggering the protests that led to the current crisis.
The health ministry says 88 people, mostly protesters, are now known to have been killed since 18 February.
Mr Turchynov, a close associate of freed former Prime Minister Yulia Tymoshenko, described forming a unity government as a "priority task".
"We don't have much time," one of the opposition leaders, Vitaly Klitschko, said as parliament began its debate.
Speaking to the BBC, he also suggested a bid for the presidency in elections scheduled for 25 May.
"I want to make Ukraine a modern European country," he said. "If I can do that through the president's position, I will do my best."
In another move, parliament voted to dismiss Foreign Minister Leonid Kozhara, an ally of Mr Yanukovych.
Yulia Tymoshenko told the crowd that "heroes never die"
Opposition leader Vitaly Klitschko: 'Protesters should stay in square'
Mr Yanukovych is refusing to officially stand down. He is last thought to have been in Kharkiv after travelling there late on Friday night.
Media reports have quoted Ukrainian officials as saying he was stopped by border police while attempting to fly to Russia aboard a private plane.
Kiev's central Independence Square remained occupied the morning after Saturday's rapid developments
Financial support
US Treasury Secretary Jack Lew discussed Ukraine with Russian Finance Minister Anton Siluanov on the sidelines of the G20 meeting in Sydney, a US official said.
Mr Lew stressed the US was ready to work "with other countries including Russia" to help Ukraine "as it implements reforms to restore economic stability and seeks to return to a path of democracy and growth".
However, Mr Siluanov has left open the question of whether Russia will pay the next - $2bn - instalment of financial help for Ukraine.
"We are planning to wait until a new government is formed and until we understand the policy of this government and then we shall make a decision," Siluanov was quoted by website Gazeta.ru as saying.
In a telephone conversation with Russian Foreign Minister Sergei Lavrov, US Secretary of State John Kerry noted the "peaceful atmosphere" prevailing in Kiev after the departure of President Yanukovych, officials said.
Russia and the US have been on opposite sides during the Ukraine crisis, which the US, along with the EU, backing the opposition.
The European Union, too, has said it stands ready to assist a new government.
The BBC's Gabriel Gatehouse takes a look around the presidential retreat
Economic and Monetary Affairs Commissioner Olli Rehn said in Sydney: "It is important that we provide a clear European perspective for the Ukrainian people".
UK Foreign Secretary William Hague told the BBC he would be speaking to Mr Lavrov on Monday.
"It's very important for us to persuade Russia that this need not be a zero-sum game," he said.
Source:BBC
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